Regulatory

Part:BBa_M36562:Design

Designed by: Ashley Henderson   Group: Stanford BIOE44 - S11   (2014-10-24)


Estrogen Response Element- operon that the estrogen receptor beta binds to


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the sequence CTG as the "NNN" in the center of the sequence design.


Source

The sequence we used was obtained from this research article. "Cooperative Estrogen Receptor Interaction with Consensus or Variant Estrogen Responsive Elements in Vitro" Carolyn M. Klinge, Franklin V. Peale, Jr., Russell Hilf, et al.

References